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KMID : 0644320010070010039
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Journal of Korean Oriental Oncology
2001 Volume.7 No. 1 p.39 ~ p.60
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Anti-metastastic Effects of ingredients of Fuzhengfangaitang
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Park Jun-Hyuk
Yu Young-Beob
Shim Bum-Sang
Choi Seung-Hun
Ahn Kyoo-Suk
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Abstract
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Fuzhengfangaitang is a prescript for inhibiting recurrence and metastasis of cancer. We had examined the anti-metatstastic effect of Fuzhengfangaitang. Furthermore, we performed the following experiments with ingredients of Fuzhengfangaitang. The purpose of this thesis is to study what ingredients of Fuzhengfangaitang have more valuable anti-cancer effects. And the results are listed below: 1. Cell Viability assay At the dose of 400g/ml, most ingredients of Fuzhengfangaitang depressed viability of ECV-304. And especially, Scutellaria barbata D. DON 50ug/ml : 53.118%, 100ug/ml : 49.092%, 200ug/ml : 43.765%, 400ug/ml : 12.747%), Polygonum bistorta L.( 50ug/ml : 45.554%, 100ug/ml : 45.554%,200ug/ml : 0.0%, 400ug/ml : 0.0%) and Psoralea corylifolia L.(50ug/ml : 86.591%,100ug/ml : 81.307%, 200ug/ml : 24.801%, 400ug/ml : 3.111%) highly depressed cell viability more than the other ingredients. (<0.05) 2. Cell Proliferation assay Proliferation assay with ingredients of Fuzhengfangaitang on ECV-304 showed that Crataegus pinnatifuda BGE (: 50ug/ml : 63.276%, 100ug/ml : 64.092%, 200ug/ml : 68.966% 400ug/ml : 38.517%, ED50=296.974ug/ml), Polygonum bistorta L.(50ug/ml : 83.981%, 100ug/ml : 86.997%, 200ug/ml : 58.780%, 400ug/ml : 26.408%), ED50=266.725ug/ml) and Psoralea corylifolia L.(50ug/ml : 103.037%, 100ug/ml : 82.529%, 200ug/ml : 2.829%, 400ug/ml : 0.998%), ED50=177.369ug/ml) depressed cell proliferation more than the other ingredients. 3. Tube Formation assay Compared with the control group, most ingredients of Fuzhengfangaitang did not remarkably inhibited the Tube Formation assay of ECV-304 at the dose of 100ug/ml. But, Polygonum bistorta L. highly inhibited the tube formation of ECV -304 at the lower dose of 50ug/ml. 4. Rat Aortic Ring assay In comparison with the control group, Scutellaria barbata D. DON., root of Polygonum bistorta L. and Psoralea corylifolia L. restricted the angiogenesis of the rat aortic ring at the dose of 100ug/ml . And the other ingredients of Fuzhengfangaitang did not restricted the angiogenesis of the rat aortic ring at that dose. Especially, Polygonum bistorta L. highly inhibited the angiogenesis of the rat aortic ring at the lower dose of 50ug/ml. From our research, the anti-angiogenic effects of the ingredients of Fuzhengfangaitang was proven. Moreover, it will be helpful for designing more effective prescription for anti angiogenesis.
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KEYWORD
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